Regulatory

Part:BBa_K909010:Design

Designed by: Deborah Huber   Group: iGEM12_ETH_Zurich   (2012-09-23)

YcgZ promoter with multiple BluR (YcgE) operator sites


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The natural YcgZ promoter and the already submitted parts (BBa_K238013 and BBa_K360041) contain only the BluR operator sites around the -35 region. We designed an improved version with double the operator sites. The operator sites around the -10 region were engineered to block the polymerase from binding by overlapping the operator sequence with the -10 sequence. The -10 region was not changed in this process so polymerase binding and performance should not be impaired.

Source

Improved version of BBa_K238013

References

The following references helped us with our design:

[http://www.ncbi.nlm.nih.gov/pubmed/19240136 Tschwori et al. "The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light response of Escherichia coli." (2009)]

[http://www.ncbi.nlm.nih.gov/pubmed/22783906 Tschwori et al. "Molecular function and potential evolution of the biofilm-modulating blue light-signalling pathway of Escherichia coli." (2012)]